Preparation of monoclonal antibody based indirect competitive ELISA for detecting 19-nortestosterone residue
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Preparation of a polyclonal antibody based heterologous indirect competitive ELISA for detecting ractopamine residue
Based on the polyclonal antibody (pAb), an indirect competitive ELISA (icELISA) has been developed for the determination of ractopamine (Rac) residue in swine and cattle. Mixed anhydride method was employed to synthesize the immunogen while 1,4-butanediol diglycidyl ether was used to synthesize the coating antigen, thus producing the heterologous sensitivity; and two female New Zealand white ra...
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Citrinin (CTN) is a hepato-nephrotoxic mycotoxin produced by fungi genera of Aspergillus, Monauscus, and Penicillium. CTN contaminates grains, fruits, juices and vegetables, and causes various toxic effects to humans and animals. It has small molecular weight, which is non-immunogenic to animals. Thus, CTN was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA), respectively, by amide ...
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ژورنال
عنوان ژورنال: Chinese Science Bulletin
سال: 2011
ISSN: 1001-6538,1861-9541
DOI: 10.1007/s11434-011-4604-y